Course Description
Laboratory practices in molecular biology, analysis, discussion, report writing, oral presentation
Curriculum
- 15 Sections
- 5 Lessons
- 20 Weeks
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- TMMO510 All VDO1
- TMMO510-1 Orientation, Laboratory safety and regulation, Ethics1
- TMMO510-2 Reagent preparations1
- TMMO510-3 Genomic DNA and plasmid extraction, quantification and analysis0
- TMMO510-4 Restriction enzyme digestion and mapping0
- TMMO510-5 Primer design, PCR and real-time PCR2
- TMMO510-6 DNA sequence analysis0
- TMMO510-7 Construction of recombinant DNA and bacterial transformation0
- TMMO510-8 Screening of bacterial transformants carrying recombinant DNA0
- TMMO510-9 RNA extraction, quantification and analysis0
- TMMO510-10 cDNA synthesis0
- TMMO510-11 Protein preparation, separation and quantification0
- TMMO510-12 Protein detection and analysis0
- TMMO510-13 Detection of targeted protein by ELISA technique0
- TMMO510-14 Cell culture and analysis by flow cytometry technique0
2 Comments
I have the question ka Aj.
What the difference between real-time PCR, qPCR, and RT-PCR?
qPCR, or quantitative PCR, is another term for real-time PCR.
RNA can be used as a template in reverse transcription PCR, or RT-PCR. RNA transcripts are commonly measured in RT-qPCR by reverse transcribing them into cDNA first, followed by qPCR.
Real-time PCR detects PCR amplification during the reaction’s exponential growth phase and is substantially more quantitative than regular RT-PCR.