student ID : 6438165
1. Describe in the group discussion; character, shape, how to collect.
1.1 Character of ovary dissection from the mosquitoes
– Parous ovary: the tracheoles are released because the mosquitoes that have been taking a blood meal and oviposited before.
– Nulliparous ovary: can see the many coiled tracheoles inside the ovary
1.2 Shape of the ovary
– can see the two oval lobes of the ovary when you pull the segments 7th and 8th out.
1.3 How to collect?
– Anesthetize mosquitoes with ether.
– Pull out the legs and wings of the mosquito.
– Use a needle to hold the mosquito with one hand.
– Use another needle to gently pull out the 7th and 8th segments of the mosquito together.
– You will see 2 bunches of ovaries attached to it.
– Then cut the ovaries out and place them on the prepared slides.
– Wait for it to dry and then observe it with a microscope.
2. How important of blood determination and mosquito parity rate?
: if we know the source of blood in the vector we can determine the relation between host and mosquitoes, host preference, host-feeding pattern, and proportion in mosquitoes. and parity rate causes us to know that areas have more or less breeding places that are connected with the transmission rate. also counting the number of ovarioles from captured mosquitoes can determine the age structure of the population.
– Methods of determination
: – The methods to determine the blood source in the mosquito can use in many ways including
• the precipitin test
• enzyme-linked immunosorbent assay (ELISA)
• multiplex PCR/DNA barcode
• Mass spectrometry
– The method of age determination including
• Parity rate in ovary dissection that each dilatation represents one gonotrophic cycle.
• Molecular markers: detect some proteins that increase or decrease after being oviposited of mosquitoes.
– Application of these 2 parameters
: can apply to surveillance and monitoring to determine vector capacity in those areas. cause to know that transmission of disease is more or less and to control the vector as soon as possible. For example, the age of female mosquitoes is determined by multiplying the number of dilatation of tracheoles in the ovary and the number of days of the gonotrophic cycle. also, the blood determination can find a host-feeding pattern and host preference in the vector.